Heating Proteins to Body Temperature Uncovers New Drug Targets

Traditionally, proteins are studied at low temperatures to maintain stability. Yet, a recent study illustrates that specific proteins exhibit high sensitivity to temperature and undergo structural changes when observed at body temperature. So heating proteins to body temperature uncovers new drug targets these findings, published today in Nature.

DateMay 15, 2024
SourceVan Andel Research Institute
SummaryA novel method for studying proteins at body temperature reveals that some proteins significantly change their structures when heated, creating new opportunities for structure-guided drug development. The study focused on a protein called TRPM4.
Biology News: Heating proteins to body temperature uncovers new drug targets

If you want to know recent biology news like heating proteins to body temperature uncovers new drug targets, then read here: Why Fasting is Not Always Good for Your Health, Specific Genomic Changes in the Monkeypox Virus Associated with Their TransmissibilityBetter View of Living Bacteria with New Mid-Infrared Nanoscopy.

About TRPM4 Protein:

  • Certain proteins alter their shape when exposed to different temperatures and reveal previously unknown binding sites for medications. These findings could transform various areas of biology by fundamentally changing the study of protein structures and their use in drug design.
  • The research centered on TRPM4, a protein essential for heart function and metabolism, including insulin release. Consequently, TRPM4 is associated with various health conditions such as stroke, heart disease, and diabetes.
  • TRPM4 is a type of protein known as a transient receptor potential (TRP) channel. It plays a crucial role in various physiological processes within the body.
  • The TRPM4 protein is a transmembrane protein, meaning it spans the cell membrane. It consists of multiple domains, including transmembrane domains that form the channel pore and regulatory domains that control its activity.
  • The TRPM4 protein is involved in regulating the flow of ions, particularly calcium ions, across cell membranes. It helps control cell signaling, which is essential for numerous functions, including heart function, metabolism, and insulin release.

How Drugs Enter into the Cell Membrane Watch Here

Experiment of How Heating Proteins to Body Temperature Uncovers New Drug Targets:

ExperimentObservationConclusion

To detect TRPM4 at body temperature, the team utilized VAI’s advanced cryo-electron microscopes (cryo-EM). These instruments enable scientists to flash-freeze proteins and create detailed images of their structures, instead of using a low-temperature sample.
This approach revealed that ligands—molecules that bind to proteins—interact with entirely different sites on TRPM4 at body temperature compared to lower temperatures.The findings of this study have extensive implications and underscore the significance of examining proteins at body temperature to accurately identify physiologically relevant drug binding sites.
Biology News: Heating proteins to body temperature uncovers new drug targets

Proteins serve as the molecular engines of the body, with their shape dictating their interactions with other molecules to carry out their functions. By unraveling protein structures, scientists can craft templates that steer the development of more potent medications and thus heating proteins to body temperature uncovers new drug targets.

FAQ:

1. What does it mean to heat proteins to body temperature?

Heating proteins to body temperature refers to subjecting proteins to the same temperature as the human body, approximately 37 degrees Celsius (98.6 degrees Fahrenheit).

2. Why is heating proteins to body temperature important?

Heating proteins to body temperature reveals how they behave under conditions similar to those inside the human body. This can provide insights into their structure and function, including how they interact with potential medications.

3. What are drug targets?

Drug targets are specific molecules, such as proteins, that medications interact with to produce a therapeutic effect. Identifying new drug targets is crucial for developing effective treatments for various diseases.

A Highly Infectious Bird Flu Virus Detected in New York City

A recent study published in the Journal of Virology, a publication of the American Society for Microbiology, found that a small number of wild birds in New York City carry highly pathogenic H5N1 avian influenza and this is a highly infectious bird flu virus.

DateMay 15, 2024
SourceAmerican Society for Microbiology
SummaryA recent study found that a small number of wild birds in New York City are carriers of highly pathogenic H5N1 avian influenza.
Biology News: Highly Infectious Bird Flu Virus Detected in New York City

If you want to know recent biology news like Highly Infectious Bird Flu Virus Detected in New York City, then read here: Why Fasting is Not Always Good for Your Health, Specific Genomic Changes in the Monkeypox Virus Associated with Their TransmissibilityBetter View of Living Bacteria with New Mid-Infrared Nanoscopy.

About H5N1:

The H5N1 virus, commonly known as avian influenza or bird flu, is a type of influenza virus that primarily affects birds but can also infect humans and other animals. It is highly pathogenic, meaning it can cause severe disease and death in poultry and has significant implications for public health and agriculture.

Transmission

H5N1 is primarily spread through direct contact with infected birds, their droppings, or contaminated environments. Human infections are rare but can occur, typically through close interaction with infected poultry. There is a concern among scientists about the potential for the virus to mutate and gain the ability to spread easily between humans, which could lead to a global pandemic.

Symptoms in Humans

In humans, H5N1 infection can cause a range of symptoms, from mild respiratory issues to severe respiratory distress and pneumonia. Common symptoms include fever, cough, sore throat, muscle aches, and in severe cases, difficulty breathing and acute respiratory distress syndrome (ARDS). The virus has a high mortality rate in humans, making it a serious public health concern.

Another Viral Infection is Here

How Highly Infectious Bird Flu Virus Detected in New York City:

CaseObservationConclusion
In the study, the researchers collected and screened 1,927 samples between January 2022 and November 2023, detecting H5N1 in six city birds from four different species.

All positive samples came from urban wildlife rehabilitation centers, highlighting their crucial role in viral surveillance.
Genetic comparison of these samples with other H5N1 viruses in a public database revealed slight differences, showing they belonged to two different genotypes.

These genotypes are a mix of Eurasian H5N1 2.3.4.4.b clade virus and local North American avian influenza viruses. New York City is a key stopover for migrating wild birds
H5N1 in city birds does not indicate the onset of a human influenza pandemic.
H5N1 has been present in New York City for about two years, and no human cases have been reported
Biology News: Highly Infectious Bird Flu Virus Detected in New York

According to the expert, it’s wise to stay vigilant and avoid contact with wildlife, including preventing pets from interacting with wild animals. If handling wildlife is necessary, it is crucial to always use safe practices when dealing with sick or injured birds or other animals because they may carry the highly infectious bird flu virus H5N1.

FAQ:

1. How can H5N1 infections be prevented?

Prevention measures include:
Monitoring and surveillance of poultry for signs of infection.
Implementing strict biosecurity measures on poultry farms.
Vaccinating poultry against H5N1.
Educating the public about risks and preventive steps, especially those working with poultry.

2. Can H5N1 be treated?

Currently, antiviral medications can be used to treat H5N1 infections in humans, but their effectiveness can vary. Early diagnosis and treatment are important for the best outcomes.

3. Is there a vaccine for H5N1?

There are vaccines for poultry to prevent H5N1, but vaccines for humans are still in development and not widely available. Research is ongoing to create effective human vaccines.
By understanding and following preventive measures, and supporting ongoing research, we can better manage and reduce the risks associated with the H5N1 virus.

Biological Classification Taxonomy Class 11 Full Chapter 2 in Gist

In life on Earth, every organism, from the tiniest microbe to the mightiest mammal, is intricately interconnected. How do we organize and categorize them? The answer lies in biological classification taxonomy. Biological classification, also known as taxonomy, organizes living organisms into hierarchical groups based on shared characteristics.

Definition of Biological Classification Taxonomy:

  • Biological classification taxonomy is a scientific discipline concerned with organizing and categorizing living organisms based on their shared characteristics and evolutionary relationships. It provides a systematic framework for naming, classifying, and studying the vast diversity of life forms on Earth.
  • Through biological classification taxonomy, organisms are grouped into hierarchical categories, from the most specific (species) to the most general (kingdom), allowing scientists to understand and communicate about the relationships between different species and groups.
  • This biological classification taxonomy system helps researchers uncover patterns of evolutionary history, biodiversity, and ecological interactions, ultimately contributing to our understanding of the natural world.

If you want to know about the first chapter, then read the article: The Living World – Full Chapter Here.

Biological Classification Taxonomy of Five Kingdom Classification:

CharactersMoneraProtistaFungi PlantaeAnimalia
Cell TypeProkaryoticEukaryoticEukaryoticEukaryoticEukaryotic
Cell WallNoncellulosic
(Polysaccharide
amino acid)
Present in
some
Present
with chitin
Present
(cellulose)
Absent
Nuclear MembraneAbsentPresentPresentPresentPresent
Body organizationCellularCellularMulticeullar/
loose tissue
Tissue/
organ
Tissue/organ/
organ system
Mode of
nutrition
Autotrophic
(chemosynthetic and
photosynthetic)
and Heterotrophic (saprophytic/parasitic)
Autotrophic
(Photosynthetic) and
Heterotrophic
Heterotrophic
(Saprophytic/
Parasitic)
Autotrophic
(Photosynthetic)
Heterotrophic
(Holozoic/
Saprophytic
etc.)
ExamplesBacteria (e.g., Escherichia coli, Bacillus subtilis)
Archaea (e.g., Methanogens, Halophiles)
Amoeba (e.g., Amoeba proteus)
Euglena (e.g., Euglena gracilis)
Paramecium (e.g., Paramecium caudatum)
Diatoms (e.g., Navicula, Cyclotella)
Mushrooms (e.g., button mushrooms, chanterelles)
Yeasts (e.g., Saccharomyces cerevisiae)
Molds (e.g., Penicillium, Aspergillus)
Lichens (symbiotic organisms consisting of fungi and algae or cyanobacteria)
Trees (e.g., oak, pine, maple)
Flowers (e.g., roses, sunflowers, tulips)
Grasses (e.g., wheat, rice, corn)
Ferns (e.g., maidenhair fern, Boston fern)
Humans (Homo sapiens sapiens)
Lions (Panthera leo)
Dogs (Canis lupus familiaris)
Insects (e.g., bees, ants, butterflies)
5 Kingdom Classification

Get the detail NEET Biology Syllabus Here.

The Biomolecules chapter is Here

Salient Features and Classification of Monera:

In biological classification taxonomy, Monera is one of the five kingdoms of biological classification, encompassing a diverse group of organisms that are characterized by their simple cellular structure and prokaryotic nature. These organisms play crucial roles in various ecosystems, from the decomposition of organic matter to nitrogen fixation.

Salient Features:

  1. Prokaryotic Cells: Monera are comprised of prokaryotic cells, which lack a true nucleus and membrane-bound organelles. Instead, their genetic material is contained within a single circular chromosome, typically located in the nucleoid region of the cell.
  2. Cellular Structure: Monerans exhibit a wide range of cellular morphologies, including cocci (spherical), bacilli (rod-shaped), and spirilla (spiral-shaped). Some species may also form colonies or filaments.
  3. Cell Wall Composition: The cell wall of Monera is primarily composed of peptidoglycan, a unique polysaccharide structure that provides structural support and protection to the cell. However, some species may lack a cell wall entirely.
  4. Metabolism: Monerans display diverse metabolic capabilities, including autotrophic and heterotrophic modes of nutrition. Autotrophic species can synthesize their own organic molecules from inorganic sources, while heterotrophic species rely on organic compounds for energy and carbon.
  5. Reproduction: Monerans reproduce asexually through binary fission, where a single cell divides into two identical daughter cells. Some species may also exchange genetic material through processes such as conjugation, transformation, or transduction.

Classification:

In biological classification taxonomy, the classification of Monera is primarily based on genetic, biochemical, and morphological characteristics. Traditionally, Monera has been divided into two main groups:

  1. Bacteria (Eubacteria): Bacteria are the most abundant and diverse group of Monerans, inhabiting virtually every environment on Earth. They exhibit a wide range of metabolic diversity, including aerobic and anaerobic respiration, nitrogen fixation, and fermentation. Bacteria are further classified based on their shape, staining characteristics (Gram-positive or Gram-negative), and metabolic properties.
  2. Archaea (Archaebacteria): Archaea are ancient prokaryotic organisms that thrive in extreme environments such as hot springs, salt flats, and deep-sea hydrothermal vents. They exhibit unique biochemical and genetic features distinct from bacteria and are classified into multiple phyla based on phylogenetic analysis.

Recent advancements in molecular biology and genomics have led to revisions in the classification of Monera, with ongoing efforts to elucidate the evolutionary relationships and diversity within this kingdom. New techniques such as metagenomics, which involve the sequencing of environmental DNA, are providing insights into the vast diversity of microbial life on Earth.

Salient Features and Classification of Protista:

In biological classification taxonomy, Protista is a diverse kingdom of eukaryotic organisms that includes a wide variety of unicellular, colonial, and multicellular organisms. These organisms exhibit a range of morphological, physiological, and ecological characteristics, making them essential components of various ecosystems.

Salient Features:

  1. Eukaryotic Cells: Protists are characterized by the presence of eukaryotic cells, which contain membrane-bound organelles including a true nucleus. This distinguishes them from prokaryotic organisms, such as bacteria and archaea.
  2. Cellular Diversity: Protists exhibit a wide range of cellular morphologies, including spherical (coccoid), rod-shaped (bacillar), spiral (spirillar), and amoeboid shapes. Some protists may form colonies or multicellular structures.
  3. Nutritional Modes: Protists display diverse modes of nutrition, including autotrophic, heterotrophic, and mixotrophic strategies. Autotrophic protists, such as algae, can photosynthesize to produce their own food, while heterotrophic protists obtain nutrients by ingesting organic matter. Mixotrophic protists exhibit a combination of autotrophic and heterotrophic characteristics.
  4. Habitats: Protists inhabit a wide range of aquatic and terrestrial environments, including freshwater and marine habitats, soil, and symbiotic relationships with other organisms. Some protists are free-living, while others are parasitic or symbiotic.
  5. Reproduction: Protists reproduce through a variety of mechanisms, including binary fission, multiple fission, budding, fragmentation, and sexual reproduction. Many protists have complex life cycles involving both sexual and asexual phases.

Classification:

In biological classification taxonomy, Protists are a diverse and polyphyletic group, meaning they do not share a common ancestor and are instead classified based on evolutionary relationships inferred from molecular, morphological, and ecological data. The classification of protists is constantly evolving as new information becomes available. However, protists are traditionally grouped into several major taxonomic groups:

  1. Algae: Algae are photosynthetic protists that can range from unicellular to multicellular forms. They are found in diverse habitats, including freshwater, marine environments, and moist terrestrial habitats. Examples include diatoms, dinoflagellates, green algae, brown algae, and red algae.
  2. Protozoa: Protozoa are heterotrophic protists that obtain nutrients by ingesting organic matter. They exhibit a wide range of morphologies and locomotion mechanisms, including flagella, cilia, and pseudopodia. Examples include amoebas, paramecia, euglenas, and ciliates.
  3. Slime Molds: Slime molds are protists that exhibit both unicellular and multicellular stages in their life cycles. They are often found in damp, decaying organic matter and play important roles in decomposition and nutrient cycling.
  4. Water Molds: Water molds, or oomycetes, are protists that resemble fungi in their filamentous morphology and mode of nutrition. They are often found in aquatic environments and are important decomposers and pathogens of plants and animals.
  5. Other Groups: In addition to the major taxonomic groups mentioned above, protists include a diverse array of organisms with unique characteristics and ecological roles. These may include colonial protists, mixotrophic organisms, and symbiotic associations such as lichens.

Salient Features and Classification of Fungi:

In biological classification taxonomy, Fungi constitute a diverse kingdom of eukaryotic organisms that play critical roles in ecosystems as decomposers, symbionts, and pathogens. They exhibit unique morphological, physiological, and ecological characteristics, making them distinct from other kingdoms of life.

Salient Features:

  1. Eukaryotic Cells: Fungi are composed of eukaryotic cells, meaning they have a true nucleus and membrane-bound organelles such as mitochondria and Golgi apparatus. This distinguishes them from prokaryotic organisms like bacteria.
  2. Heterotrophic Nutrition: Fungi are heterotrophs, meaning they obtain their nutrients by absorbing organic matter from their environment. They secrete enzymes that break down complex organic molecules into simpler forms, which are then absorbed through their cell walls.
  3. Cell Wall Composition: Fungi have cell walls primarily composed of chitin, a complex polysaccharide that provides structural support and protection. This sets them apart from other eukaryotic organisms, such as plants and animals, which have cell walls made of cellulose or other materials.
  4. Morphological Diversity: Fungi exhibit a wide range of morphologies, including multicellular filamentous structures called hyphae, which collectively form a network known as mycelium. Some fungi produce specialized reproductive structures such as mushrooms, while others exist as yeasts or single-celled forms.
  5. Reproduction: Fungi reproduce through a combination of sexual and asexual means. Sexual reproduction involves the fusion of specialized reproductive structures called gametes, while asexual reproduction occurs through the production of spores, which are dispersed and germinate to form new fungal colonies.

Classification:

In biological classification taxonomy, the Fungi are classified into several major taxonomic groups based on their morphological characteristics, reproductive strategies, and genetic relationships. The classification of fungi is complex and continues to evolve with advances in molecular biology and phylogenetics. However, the major taxonomic groups include:

  1. Phylum Zygomycota: Zygomycetes are characterized by their production of thick-walled resting structures called zygospores, which result from the fusion of specialized hyphae. They include bread molds and other saprophytic fungi.
  2. Phylum Ascomycota: Ascomycetes, or sac fungi, produce sexual spores called ascospores within sac-like structures called asci. They include diverse organisms such as yeasts, molds, truffles, and morels.
  3. Phylum Basidiomycota: Basidiomycetes, or club fungi, produce sexual spores called basidiospores on specialized club-shaped structures called basidia. They include mushrooms, puffballs, rusts, and smuts.
  4. Phylum Glomeromycota: Glomeromycetes form arbuscular mycorrhizal symbioses with the roots of most terrestrial plants, playing essential roles in nutrient uptake and plant health.
  5. Phylum Chytridiomycota: Chytrids are aquatic or soil-dwelling fungi characterized by their flagellated reproductive cells. Some chytrid species are parasites of plants, algae, or animals, while others are saprobes.
  6. Phylum Mycetozoa: Mycetozoans, or slime molds, are unique fungi that exhibit both animal-like and fungus-like characteristics. They include plasmodial slime molds and cellular slime molds.

Let’s Visualize The Chapter Biological Classification Taxonomy

Kingdom Plantae:

  • In biological classification taxonomy, the kingdom Plantae encompasses all eukaryotic organisms containing chlorophyll, commonly referred to as plants.
  • Some members of this kingdom exhibit partial heterotrophy, such as insectivorous plants or parasites. Examples of insectivorous plants include bladderwort and Venus flytrap, while Cuscuta serves as a parasite.
  • Plant cells display a eukaryotic structure characterized by prominent chloroplasts and a cell wall primarily composed of cellulose.
  • Plantae comprises algae, bryophytes, pteridophytes, gymnosperms, and angiosperms.
  • The life cycle of plants involves two distinct phases: the diploid sporophytic and the haploid gametophytic phases, which alternate with each other.
  • The duration of these phases and whether they are independent or reliant on other organisms vary among different plant groups. This phenomenon is known as alternation of generations.

Kingdom Animalia:

  • In biological classification taxonomy, this kingdom comprises multicellular heterotrophic eukaryotic organisms whose cells lack cell walls.
  • They rely directly or indirectly on plants for sustenance, digesting their food internally and storing reserves as glycogen or fat.
  • Their nutritional mode is holozoic, involving the ingestion of food.
  • These organisms follow a defined growth pattern, reaching adulthood with distinct shapes and sizes.
  • Higher forms exhibit sophisticated sensory and neuromotor mechanisms, with many capable of locomotion.
  • Sexual reproduction in this kingdom occurs through the copulation of male and female individuals, followed by embryonic development.

Lichens, Viruses, and Viroids:

In this living world, there exist microorganisms that defy conventional classification and challenge our understanding of biological diversity. Lichens, viruses, and viroids are three such entities that hold profound significance in ecology, medicine, and molecular biology.

Lichens

Lichens are remarkable organisms that often go unnoticed in the natural world, yet they play crucial roles in various ecosystems and have captivated scientists and nature enthusiasts alike for centuries. In this article, we will delve into the salient features and classification of lichens, shedding light on their fascinating biology and diversity.

Salient Features of Lichens:

  1. Symbiotic Nature: One of the most distinctive features of lichens is their symbiotic relationship between a fungus (the mycobiont) and a photosynthetic partner (the photobiont), typically green algae or cyanobacteria. This symbiosis allows lichens to thrive in a wide range of environments, from barren deserts to harsh polar regions.
  2. Thallus Structure: Lichens exhibit a variety of growth forms, collectively known as thalli, which can range from crustose (crust-like) to foliose (leaf-like) and fruticose (shrub-like). These growth forms are adapted to different environmental conditions and play important roles in nutrient acquisition, water retention, and reproduction.
  3. Environmental Tolerance: Lichens are known for their remarkable tolerance to extreme environmental conditions, including drought, high levels of ultraviolet radiation, and pollution. They are often the first organisms to colonize bare rock or soil, initiating the process of primary succession in ecosystems.
  4. Chemical Defense: Lichens produce a variety of secondary metabolites, including pigments, acids, and antibiotics, which serve as chemical defenses against herbivores, pathogens, and competing organisms. These compounds also contribute to the diverse colors and textures observed in lichens.
  5. Reproductive Strategies: Lichens reproduce through a combination of sexual and asexual means. Sexual reproduction involves the formation of fungal spores or algal cells, which can fuse to form a new lichen thallus. Asexual reproduction occurs through fragmentation, where portions of the thallus break off and establish new colonies.

Classification of Lichens:

Lichens are classified based on the dominant fungal partner (the mycobiont) and the growth form of the thallus. The most common groups of lichens include:

  1. Crustose Lichens: These lichens have a crust-like thallus that is tightly attached to the substrate, such as rocks or bark. Examples include species of the genera Lecanora and Caloplaca.
  2. Foliose Lichens: Foliose lichens have a leaf-like thallus with distinct upper and lower surfaces. They are often loosely attached to the substrate and can be easily detached. Examples include species of the genera Parmelia and Lobaria.
  3. Fruticose Lichens: Fruticose lichens have a shrub-like or branching thallus that is typically erect or pendant. These lichens can be found growing on soil, rocks, or other substrates. Examples include species of the genera Usnea and Cladonia.
  4. Squamulose Lichens: Squamulose lichens have a thallus composed of small, scale-like structures called squamules. They are often found growing on soil or rocks in arid or semi-arid environments. Examples include species of the genera Psora and Cladia.

Ecological Significance:

Lichens play vital roles in ecosystems as pioneers in primary succession, contributing to soil formation, nitrogen fixation, and habitat creation. They serve as food sources for a variety of organisms, including insects, birds, and mammals, and are important indicators of environmental health and air quality.

Examples of Lichens:

  1. Reindeer Lichen (Cladonia rangiferina): Found in Arctic and subarctic regions, this lichen forms dense mats resembling reindeer antlers. It is an important food source for caribou and reindeer during harsh winters.
  2. British Soldier Lichen (Cladonia cristatella): Named for its red caps resembling British soldiers’ uniforms, this lichen is commonly found on tree bark and rocks in forests across North America.
  3. Old Man’s Beard (Usnea spp.): This distinctive lichen hangs from tree branches like grayish-green beards, giving it its common name. It is found in various habitats worldwide and is used in traditional medicine.
  4. Pixie Cup Lichen (Cladonia pyxidata): Recognizable by its cup-shaped fruiting bodies, this lichen grows on soil, rocks, and tree bark in forests and heathlands. It is widespread across Europe and North America.
  5. Powdered Sunshine Lichen (Xanthoria spp.): This bright yellow or orange lichen is commonly found on rocks, tree bark, and man-made structures in sunny, exposed habitats. It is tolerant of pollution and is often used as an indicator of air quality.

Viruses

Viruses are fascinating microorganisms that straddle the line between living and non-living entities. Despite their simplicity, they wield immense power in shaping ecosystems and influencing the course of life on Earth.

Salient Features of Viruses:

  1. Submicroscopic Size: Viruses are incredibly small, ranging in size from 20 to 300 nanometers. They are not visible under a light microscope and require specialized techniques such as electron microscopy for visualization.
  2. Genetic Material: Viruses contain genetic material, either DNA or RNA, surrounded by a protein coat called a capsid. Some viruses also possess additional structures such as an envelope derived from the host cell membrane.
  3. Host Dependence: Viruses are obligate intracellular parasites, meaning they can only replicate inside host cells. They lack the cellular machinery necessary for metabolism and reproduction and must hijack the host cell’s machinery to replicate.
  4. Diverse Shapes: Viruses exhibit a wide range of shapes, including helical, icosahedral, and complex structures. Their morphology is determined by the arrangement of viral proteins and genetic material within the capsid.
  5. Modes of Transmission: Viruses employ diverse strategies for transmission, including direct contact, respiratory droplets, fecal-oral route, and vector-borne transmission by arthropods. Understanding the mechanisms of viral transmission is crucial for controlling and preventing viral outbreaks.

Classification of Viruses:

Viruses are classified based on various criteria, including their genetic material, structure, mode of replication, and host specificity. The International Committee on Taxonomy of Viruses (ICTV) classifies viruses into several orders, families, genera, and species. Common taxonomic groups of viruses include:

  1. DNA Viruses: These viruses contain DNA as their genetic material and include families such as Herpesviridae, Adenoviridae, and Papillomaviridae.
  2. RNA Viruses: RNA viruses contain RNA as their genetic material and are further classified into positive-sense RNA viruses (e.g., Picornaviridae, Flaviviridae), negative-sense RNA viruses (e.g., Paramyxoviridae, Orthomyxoviridae), and double-stranded RNA viruses (e.g., Reoviridae).
  3. Retroviruses: Retroviruses contain RNA as their genetic material but replicate through a reverse transcription process to produce DNA. Examples include Human Immunodeficiency Virus (HIV) and Human T-cell Leukemia Virus (HTLV).

Examples of Viruses:

  1. Influenza Virus: The influenza virus belongs to the Orthomyxoviridae family and is responsible for seasonal flu outbreaks worldwide. It exhibits high mutation rates, leading to the emergence of new strains and the need for annual vaccination.
  2. Human Immunodeficiency Virus (HIV): HIV is a retrovirus that causes Acquired Immunodeficiency Syndrome (AIDS), a condition characterized by the progressive weakening of the immune system. Despite advances in treatment, HIV/AIDS remains a significant global health challenge.
  3. Herpes Simplex Virus (HSV): HSV belongs to the Herpesviridae family and is responsible for oral and genital herpes infections. It establishes latent infections in nerve cells, leading to recurrent outbreaks of painful sores.
  4. Hepatitis B Virus (HBV): HBV is a DNA virus that infects the liver, causing acute and chronic hepatitis. It is transmitted through contact with infected blood or bodily fluids and can lead to severe liver damage, including cirrhosis and liver cancer.
  5. Ebola Virus: Ebola virus belongs to the Filoviridae family and causes severe hemorrhagic fever in humans and non-human primates. It is transmitted through direct contact with infected bodily fluids and has a high mortality rate during outbreaks.

Viroids

Viroids are intriguing entities in the realm of microbiology, representing a unique group of infectious agents with a minimalistic structure and significant implications for plant health and agriculture.

Salient Features of Viroids:

  1. Minimalistic Structure: Viroids are small, circular RNA molecules that lack a protein coat (capsid). They typically range in size from a few hundred to over a thousand nucleotides and are among the smallest infectious agents known to science.
  2. No Protein Coding Capacity: Unlike viruses, viroids do not encode any proteins and rely entirely on host cellular machinery for replication and propagation. They contain a single-stranded RNA genome that folds into complex secondary structures critical for their biological activity.
  3. Pathogenic Potential: Viroids are pathogens of plants, causing a variety of diseases characterized by stunting, leaf curling, yellowing, and necrosis. They infect a wide range of plant species, including crop plants such as potatoes, tomatoes, citrus, and avocado, leading to significant economic losses in agriculture.
  4. Mechanism of Infection: Viroids enter plant cells through wounds or natural openings and replicate in the nucleus or chloroplasts using host enzymes. They induce changes in gene expression, disrupt cellular processes, and trigger immune responses in infected plants.

Classification of Viroids:

Viroids are classified into two families based on their structure, sequence homology, and host range:

  1. Pospiviroidae: This family includes viroids with a rod-like secondary structure and conserved central regions. Examples of pospiviroids include Potato spindle tuber viroid (PSTVd) and Tomato chlorotic dwarf viroid (TCDVd).
  2. Avsunviroidae: Avsunviroids have a branched secondary structure with conserved central regions and variable terminal regions. The type species in this family is Avocado sunblotch viroid (ASBVd).

Examples of Viroids:

  1. Potato Spindle Tuber Viroid (PSTVd): PSTVd is one of the best-characterized viroids and causes spindle tuber disease in potatoes. It is transmitted through infected tubers and leads to reduced yields and tuber quality.
  2. Citrus Exocortis Viroid (CEVd): CEVd infects citrus trees and is responsible for citrus exocortis disease, characterized by stunted growth, bark scaling, and reduced fruit yield. It is transmitted through grafting and mechanical contact.
  3. Tomato Chlorotic Dwarf Viroid (TCDVd): TCDVd infects tomato plants and causes chlorotic dwarfism, leaf curling, and reduced fruit size. It is transmitted through mechanical contact and seed transmission.
  4. Apple Scar Skin Viroid (ASSVd): ASSVd infects apple trees and leads to the development of scarred lesions on fruit skins, reducing their marketability. It is transmitted through infected budwood and grafting.
  5. Hop Stunt Viroid (HSVd): HSVd infects hop plants and causes stunting, chlorosis, and reduction in cone production. It is transmitted through infected plant material and contributes to economic losses in the hop industry.

The biological classification taxonomy serves as a foundational tool in our exploration and understanding of the intricate web of life on Earth. By systematically organizing and categorizing living organisms based on their shared characteristics and evolutionary relationships, taxonomy provides a roadmap for navigating the complexities of biodiversity.

FAQ on Biological Classification Taxonomy:

1. How does biological classification taxonomy contribute to our understanding of biodiversity?

Taxonomy helps scientists organize and study the diversity of life, allowing them to identify patterns of evolutionary history, biodiversity hotspots, and ecological relationships between different organisms and ecosystems.

2. What are some challenges in biological classification taxonomy?

Challenges in taxonomy include defining species boundaries, reconciling conflicting classification schemes, and integrating new discoveries and technologies into existing taxonomic frameworks. Additionally, the rapid pace of biodiversity loss underscores the urgency of taxonomy in cataloging and conserving Earth’s biological diversity.

3. How does biological classification taxonomy impact other scientific disciplines?

Taxonomy provides a foundation for various scientific disciplines, including ecology, evolutionary biology, biogeography, conservation biology, and agriculture. It informs research on ecosystem dynamics, species interactions, evolutionary processes, and the sustainable management of natural resources.

CSIR NET Life Sciences Syllabus 2024 | CSIR NET Syllabus Life Science 2024

The CSIR NET (Council of Scientific and Industrial Research National Eligibility Test) is a prestigious examination conducted in India for individuals aspiring to pursue a career in the field of Life Sciences. The CSIR NET Life Sciences syllabus for the exam is designed to assess candidates’ knowledge and understanding of various disciplines within the life sciences domain. This article provides a comprehensive overview of the CSIR NET Life Sciences syllabus to help aspiring candidates prepare effectively and increase their chances of success.

CSIR NET Life Sciences Syllabus Overview

Section Name Description
1. Molecules and their Interaction Relevant to BiologyThis section of the CSIR NET Life Sciences syllabus focuses on the fundamental concepts of biochemistry, including the structure and function of biomolecules, enzymology, and metabolism. Key topics covered in this section include amino acids, proteins, nucleic acids, carbohydrates, lipids, and their interactions within biological systems.
2. Cellular OrganizationThis section of the CSIR NET Life Sciences syllabus delves into the organization and functioning of cells. It covers topics such as cell structure, cell cycle, cell division, and cell signaling. Candidates should have a clear understanding of cellular components, organelles, and their roles in cellular processes.
3.Fundamental ProcessesThe fundamental processes section of the CSIR NET Life Sciences syllabus emphasizes the molecular mechanisms underlying various biological phenomena. Topics covered in this section include DNA replication, transcription, translation, gene expression, and regulation. Understanding the principles of genetics and molecular biology is crucial for success in this section.
4. Cell Communication and Cell SignalingThis section of the CSIR NET Life Sciences syllabus focuses on intercellular communication and signaling mechanisms. Candidates are expected to have knowledge of various signaling pathways, signal transduction, and cellular responses to external stimuli. Topics covered may include hormones, growth factors, neurotransmitters, and their roles in cellular signaling.
5. Developmental BiologyThis section of the CSIR NET Life Sciences syllabus explores the processes and mechanisms that govern the development of multicellular organisms. Topics covered may include embryogenesis, organogenesis, stem cells, and differentiation. A comprehensive understanding of developmental biology is essential to excel in this section.
6. System Physiology – Plant and 7. AnimalThis section of the CSIR NET Life Sciences syllabus deals with the physiology of plants and animals. It covers topics such as homeostasis, organ systems, transport processes, and physiological adaptations. Candidates should have a sound knowledge of plant and animal physiology and their respective mechanisms.
8. Inheritance BiologyThis section of the CSIR NET Life Sciences syllabus focuses on the principles of inheritance and genetic variation. Topics covered may include Mendelian genetics, population genetics, chromosomal inheritance, and genetic disorders. A strong foundation in genetics is crucial for success in this section.
9. Diversity of Life FormsThis section of the CSIR NET Life Sciences syllabus explores the diversity of life on Earth, including classification and taxonomy. Candidates should have knowledge of different groups of organisms, their evolutionary relationships, and their ecological significance. Topics covered may include bacteria, fungi, plants, animals, and viruses.
10. Ecology and EnvironmentThis section of the CSIR NET Life Sciences syllabus emphasizes the interrelationships between organisms and their environment. Topics covered may include ecological principles, ecosystem dynamics, biodiversity conservation, and environmental pollution. Understanding ecological concepts and environmental issues is essential for success in this section.
11. Evolution and BehaviorThis section of the CSIR NET Life Sciences syllabus explores the processes of genetic variation, natural selection, and adaptation, along with the genetic and ecological factors that influence the evolution of behavior.
12. Applied BiologyThis section of the CSIR NET Life Sciences syllabus covers a range of topics that highlight the relevance of biology in solving real-world problems and addressing societal needs. The syllabus provides an overview of the following areas
13. Methods in BiologyThis section of the CSIR NET Life Sciences syllabus focuses on the various experimental and analytical techniques employed in biological research.

Detailed CSIR NET Life Sciences Syllabus

1. MOLECULES AND THEIR INTERACTION RELAVENT TO BIOLOGY

A. Structure of atoms, molecules and chemical bonds.

Let’s go to the structures.

B. Composition, structure, and function of biomolecules (carbohydrates, lipids, proteins, nucleic acids, and vitamins).

Let’s go to the structures.

C. Stabilizing interactions (Van der Waals, electrostatic, hydrogen bonding, hydrophobic interaction, etc.).

D Principles of biophysical chemistry (pH, buffer, reaction kinetics, thermodynamics, colligative properties).

E. Bioenergetics, glycolysis, oxidative phosphorylation, coupled reaction, group transfer, biological energy transducers.

F. Principles of catalysis, enzymes and enzyme kinetics, enzyme regulation, mechanism of enzyme catalysis, isozymes

G. Conformation of proteins (Ramachandran plot, secondary structure, domains, motif and folds).

H. Conformation of nucleic acids (helix (A, B, Z), t-RNA, micro-RNA).

Let’s go to the structures.

I. Stability of proteins and nucleic acids.

J. Metabolism of carbohydrates, lipids, amino acids nucleotides and vitamins.

2. CELLULAR ORGANIZATION

A) Membrane structure and function (Structure of model membrane, lipid bilayer and membrane protein diffusion, osmosis, ion channels, active transport, membrane pumps, mechanism of sorting and regulation of intracellular transport, electrical properties of membranes).

B) Structural organization and function of intracellular organelles (Cell wall, nucleus, mitochondria, Golgi bodies, lysosomes, endoplasmic reticulum, peroxisomes, plastids, vacuoles, chloroplast, structure & function of cytoskeleton and its role in motility).

C) Organization of genes and chromosomes (Operon, unique and repetitive DNA, interrupted genes, gene families, structure of chromatin and chromosomes, heterochromatin, euchromatin, transposons).

D) Cell division and cell cycle (Mitosis and meiosis, their regulation, steps in cell cycle, regulation and control of cell cycle).

E) Microbial Physiology (Growth yield and characteristics, strategies of cell division, stress response)

3. FUNDAMENTAL PROCESSES

A) DNA replication, repair and recombination (Unit of replication, enzymes involved, replication origin and replication fork, fidelity of replication, extrachromosomal replicons, DNA damage and repair mechanisms, homologous and site-specific recombination).

B) RNA synthesis and processing (transcription factors and machinery, formation of initiation complex, transcription activator and repressor, RNA polymerases, capping, elongation, and termination, RNA processing, RNA editing, splicing, and polyadenylation, structure and function of different types of RNA, RNA transport).

C) Protein synthesis and processing (Ribosome, formation of initiation complex, initiation factors and their regulation, elongation and elongation factors, termination, genetic code, aminoacylation of tRNA, tRNA-identity, aminoacyl tRNA synthetase, and translational proof-reading, translational inhibitors, Post- translational modification of proteins).

D) Control of gene expression at transcription and translation level (regulating the expression of phages, viruses, prokaryotic and eukaryotic genes, role of chromatin in gene expression and gene silencing).

4. Cell communication and cell signaling

A) Host parasite interaction Recognition and entry processes of different pathogens like bacteria, viruses into animal and plant host cells, alteration of host cell behavior by pathogens, virus-induced cell transformation, pathogen-induced diseases in animals and plants, cell-cell fusion in both normal and abnormal cells.

B) Cell signaling Hormones and their receptors, cell surface receptor, signaling through G-protein coupled receptors, signal transduction pathways, second messengers, regulation of signaling pathways, bacterial and plant two component systems, light signaling in plants, bacterial chemotaxis and quorum sensing.

C) Cellular communication Regulation of hematopoiesis, general principles of cell communication, cell adhesion and roles of different adhesion molecules, gap junctions, extracellular matrix, integrins, neurotransmission and its regulation.

D) Cancer Genetic rearrangements in progenitor cells, oncogenes, tumor suppressor genes, cancer and the cell cycle, virus-induced cancer, metastasis, interaction of cancer cells with normal cells, apoptosis, therapeutic interventions of uncontrolled cell growth.

E) Innate and adaptive immune system Cells and molecules involved in innate and adaptive immunity, antigens, antigenicity and immunogenicity. B and T cell epitopes, structure and function of antibody molecules. generation of antibody diversity, monoclonal antibodies, antibody engineering, antigen-antibody interactions, MHC molecules, antigen processing and presentation, activation and differentiation of B and T cells, B and T cell receptors, humoral and cell-mediated immune responses, primary and secondary immune modulation, the complement system, Toll-like receptors, cell-mediated effector functions, inflammation, hypersensitivity and autoimmunity, immune response during bacterial (tuberculosis), parasitic (malaria) and viral (HIV) infections, congenital and acquired immunodeficiencies, vaccines.

5. DEVELOPMENTAL BIOLOGY

A) Basic concepts of development : Potency, commitment, specification, induction, competence, determination and differentiation; morphogenetic gradients; cell fate and cell lineages; stem cells; genomic equivalence and the cytoplasmic determinants; imprinting; mutants and transgenics in analysis of development

B) Gametogenesis, fertilization and early development: Production of gametes, cell surface molecules in sperm-egg recognition in animals; embryo sac development and double fertilization in plants; zygote formation, cleavage, blastula formation, embryonic fields, gastrulation and formation of germ layers in animals; embryogenesis, establishment of symmetry in plants; seed formation and germination.

C) Morphogenesis and organogenesis in animals : Cell aggregation and differentiation in Dictyostelium; axes and pattern formation in Drosophila, amphibia and chick; organogenesis – vulva formation in Caenorhabditis elegans, eye lens induction, limb development and regeneration in vertebrates; differentiation of neurons, post embryonic development- larval formation, metamorphosis; environmental regulation of normal development; sex determination.

D) Morphogenesis and organogenesis in plants: Organization of shoot and root apical meristem; shoot and root development; leaf development and phyllotaxy; transition to flowering, floral meristems and floral development in Arabidopsis and Antirrhinum

E) Programmed cell death, aging and senescence

6. SYSTEM PHYSIOLOGY – PLANT

A. Photosynthesis – Light harvesting complexes; mechanisms of electron transport; photoprotective mechanisms; CO2 fixation-C3, C4 and CAM pathways.

B. Respiration and photorespiration – Citric acid cycle; plant mitochondrial electron transport and ATP synthesis; alternate oxidase; photorespiratory pathway.

C. Nitrogen metabolism – Nitrate and ammonium assimilation; amino acid biosynthesis.

D. Plant hormones – Biosynthesis, storage, breakdown and transport; physiological effects and mechanisms of action.

E. Sensory photobiology – Structure, function and mechanisms of action of phytochromes, cryptochromes and phototropins; stomatal movement; photoperiodism and biological clocks.

F. Solute transport and photoassimilate translocation – uptake, transport and translocation of water, ions, solutes and macromolecules from soil, through cells, across membranes, through xylem and phloem; transpiration; mechanisms of loading and unloading of photoassimilates.

G. Secondary metabolites – Biosynthesis of terpenes, phenols and nitrogenous compounds and their roles.

H. Stress physiology – Responses of plants to biotic (pathogen and insects) and abiotic (water, temperature and salt) stresses.

7. SYSTEM PHYSIOLOGY – ANIMAL

A. Blood and circulation – Blood corpuscles, haemopoiesis and formed elements, plasma function, blood volume, blood volume regulation, blood groups, haemoglobin, immunity, haemostasis.

B. Cardiovascular System: Comparative anatomy of heart structure, myogenic heart, specialized tissue, ECG – its principle and significance, cardiac cycle, heart as a pump, blood pressure, neural and chemical regulation of all above.

C. Respiratory system – Comparison of respiration in different species, anatomical considerations, transport of gases, exchange of gases, waste elimination, neural and chemical regulation of respiration.

D. Nervous system – Neurons, action potential, gross neuroanatomy of the brain and spinal cord, central and peripheral nervous system, neural control of muscle tone and posture.

E. Sense organs – Vision, hearing and tactile response.

F. Excretory system – Comparative physiology of excretion, kidney, urine formation, urine concentration, waste elimination, micturition, regulation of water balance, blood volume, blood pressure, electrolyte balance, acid-base balance.

G. Thermoregulation – Comfort zone, body temperature – physical, chemical, neural regulation, acclimatization.

H. Stress and adaptation

I. Digestive system – Digestion, absorption, energy balance, BMR.

J. Endocrinology and reproduction – Endocrine glands, basic mechanism of hormone action, hormones and diseases; reproductive processes, gametogenesis, ovulation, neuroendocrine regulation

8. INHERITANCE BIOLOGY

A) Mendelian principles : Dominance, segregation, independent assortment.

B) Concept of gene : Allele, multiple alleles, pseudoallele, complementation tests

C) Extensions of Mendelian principles : Codominance, incomplete dominance, gene interactions, pleiotropy, genomic imprinting, penetrance and expressivity, phenocopy, linkage and crossing over, sex linkage, sex limited and sex influenced characters.

D) Gene mapping methods : Linkage maps, tetrad analysis, mapping with molecular markers, mapping by using somatic cell hybrids, development of mapping population in plants.

E) Extra chromosomal inheritance : Inheritance of Mitochondrial and chloroplast genes, maternal inheritance.

F) Microbial genetics : Methods of genetic transfers – transformation, conjugation, transduction and sex-duction, mapping genes by interrupted mating, fine structure analysis of genes.

G) Human genetics : Pedigree analysis, lod score for linkage testing, karyotypes, genetic disorders.

H) Quantitative genetics : Polygenic inheritance, heritability and its measurements, QTL mapping.

I) Mutation : Types, causes and detection, mutant types – lethal, conditional, biochemical, loss of function, gain of function, germinal verses somatic mutants, insertional mutagenesis.

J) Structural and numerical alterations of chromosomes : Deletion, duplication, inversion, translocation, ploidy and their genetic implications.

K) Recombination : Homologous and non-homologous recombination including transposition.

9. DIVERSITY OF LIFE FORMS

A. Principles & methods of taxonomy: Concepts of species and hierarchical taxa, biological nomenclature, classical & quantititative methods of taxonomy of plants, animals and microorganisms.

B. Levels of structural organization: Unicellular, colonial and multicellular forms. Levels of organization of tissues, organs & systems. Comparative anatomy, adaptive radiation, adaptive modifications.

C. Outline classification of plants, animals & microorganisms: Important criteria used for classification in each taxon. Classification of plants, animals and microorganisms. Evolutionary relationships among taxa.

D. Natural history of Indian subcontinent: Major habitat types of the subcontinent, geographic origins and migrations of species. Comman Indian mammals, birds. Seasonality and phenology of the subcontinent.

E. Organisms of health & agricultural importance: Common parasites and pathogens of humans, domestic animals and crops.

F. Organisms of conservation concern: Rare, endangered species. Conservation strategies.

10. ECOLOGICAL PRINCIPLES

The Environment: Physical environment; biotic environment; biotic and abiotic interactions.

Habitat and Niche: Concept of habitat and niche; niche width and overlap; fundamental and realized niche; resource partitioning; character displacement.

Population Ecology: Characteristics of a population; population growth curves; population regulation; life history strategies (r and K selection); concept of metapopulation – demes and dispersal, interdemic extinctions, age structured populations.

Species Interactions: Types of interactions, interspecific competition, herbivory, carnivory, pollination, symbiosis.

Community Ecology: Nature of communities; community structure and attributes; levels of species diversity and its measurement; edges and ecotones.

Ecological Succession: Types; mechanisms; changes involved in succession; concept of climax.

Ecosystem Ecology: Ecosystem structure; ecosystem function; energy flow and mineral cycling (C,N,P); primary production and decomposition; structure and function of some Indian ecosystems: terrestrial (forest, grassland) and aquatic (fresh water, marine, eustarine). Biogeography: Major terrestrial biomes; theory of island biogeography; biogeographical zones of India.

Applied Ecology: Environmental pollution; global environmental change; biodiversity: status, monitoring and documentation; major drivers of biodiversity change; biodiversity management approaches.

Conservation Biology: Principles of conservation, major approaches to management, Indian case studies on conservation/management strategy (Project Tiger, Biosphere reserves).

11. EVOLUTION AND BEHAVIOUR

A. Emergence of evolutionary thoughts Lamarck; Darwin–concepts of variation, adaptation, struggle, fitness and natural selection; Mendelism; Spontaneity of mutations; The evolutionary synthesis.

B. Origin of cells and unicellular evolution: Origin of basic biological molecules; Abiotic synthesis of organic monomers and polymers; Concept of Oparin and Haldane; Experiement of Miller (1953); The first cell; Evolution of prokaryotes; Origin of eukaryotic cells; Evolution of unicellular eukaryotes; Anaerobic metabolism, photosynthesis and aerobic metabolism.

C. Paleontology and Evolutionary History: The evolutionary time scale; Eras, periods and epoch; Major events in the evolutionary time scale; Origins of unicellular and multi cellular organisms; Major groups of plants and animals; Stages in primate evolution including Homo.

D. Molecular Evolution: Concepts of neutral evolution, molecular divergence and molecular clocks; Molecular tools in phylogeny, classification and identification; Protein and nucleotide sequence analysis; origin of new genes and proteins; Gene duplication and divergence.

E. The Mechanisms: Population genetics – Populations, Gene pool, Gene frequency; Hardy-Weinberg Law; concepts and rate of change in gene frequency through natural selection, migration and random genetic drift; Adaptive radiation; Isolating mechanisms; Speciation; Allopatricity and Sympatricity; Convergent evolution; Sexual selection; Co-evolution.

F. Brain, Behavior and Evolution: Approaches and methods in study of behavior; Proximate and ultimate causation; Altruism and evolution-Group selection, Kin selection, Reciprocal altruism; Neural basis of learning, memory, cognition, sleep and arousal; Biological clocks; Development of behavior; Social communication; Social dominance; Use of space and territoriality; Mating systems, Parental investment and Reproductive success; Parental care; Aggressive behavior; Habitat selection and optimality in foraging; Migration, orientation and navigation; Domestication and behavioral changes.

12. APPLIED BIOLOGY

A. Microbial fermentation and production of small and macro molecules.

B. Application of immunological principles, vaccines, diagnostics. Tissue and cell culture methods for plants and animals.

C. Transgenic animals and plants, molecular approaches to diagnosis and strain identification.

D. Genomics and its application to health and agriculture, including gene therapy.

E. Bioresource and uses of biodiversity.

F. Breeding in plants and animals, including marker – assisted selection

G. Bioremediation and phytoremediation

H. Biosensors

13. METHODS IN BIOLOGY

A. Molecular Biology and Recombinant DNA methods: Isolation and purification of RNA , DNA (genomic and plasmid) and proteins, different separation methods. Analysis of RNA, DNA and proteins by one and two dimensional gel electrophoresis, Isoelectric focusing gels. Molecular cloning of DNA or RNA fragments in bacterial and eukaryotic systems. Expression of recombinant proteins using bacterial, animal and plant vectors. Isolation of specific nucleic acid sequences Generation of genomic and cDNA libraries in plasmid, phage, cosmid, BAC and YAC vectors. In vitro mutagenesis and deletion techniques, gene knock out in bacterial and eukaryotic organisms. Protein sequencing methods, detection of post translation modification of proteins. DNA sequencing methods, strategies for genome sequencing. Methods for analysis of gene expression at RNA and protein level, large scale expression, such as micro array based techniques Isolation, separation and analysis of carbohydrate and lipid molecules RFLP, RAPD and AFLP techniques

B. Histochemical and Immunotechniques Antibody generation, Detection of molecules using ELISA, RIA, western blot, immunoprecipitation, fluocytometry and immunofluorescence microscopy, detection of molecules in living cells, in situ localization by techniques such as FISH and GISH.

C Biophysical Method: Molecular analysis using UV/visible, fluorescence, circular dichroism, NMR and ESR spectroscopy Molecular structure determination using X-ray diffraction and NMR, Molecular analysis using light scattering, different types of mass spectrometry and surface plasma resonance methods.

D Statisitcal Methods: Measures of central tendency and dispersal; probability distributions (Binomial, Poisson and normal); Sampling distribution; Difference between parametric and non-parametric statistics; Confidence Interval; Errors; Levels of significance; Regression and Correlation; t-test; Analysis of variance; X2 test;; Basic introduction to Muetrovariate statistics, etc.

E. Radiolabeling techniques: Detection and measurement of different types of radioisotopes normally used in biology, incorporation of radioisotopes in biological tissues and cells, molecular imaging of radioactive material, safety guidelines.

F. Microscopic techniques: Visulization of cells and subcellular components by light microscopy, resolving powers of different microscopes, microscopy of living cells, scanning and transmission microscopes, different fixation and staining techniques for EM, freeze-etch and freeze fracture methods for EM, image processing methods in microscopy.

G. Electrophysiological methods: Single neuron recording, patch-clamp recording, ECG, Brain activity recording, lesion and stimulation of brain, pharmacological testing, PET, MRI, fMRI, CAT .

H. Methods in field biology: Methods of estimating population density of animals and plants, ranging patterns through direct, indirect and remote observations, sampling methods in the study of behavior, habitat characterization: ground and remote sensing methods.

A thorough understanding of the CSIR NET Life Sciences syllabus is the first step towards achieving success in this highly competitive examination. By familiarizing themselves with the topics outlined in the syllabus, aspiring candidates can effectively plan their preparation strategies and focus on areas that require more attention. It is also crucial to supplement syllabus study with practice tests, previous year question papers, and reference books to enhance knowledge and improve exam performance. With dedication, perseverance, and a comprehensive understanding of the syllabus, aspiring candidates can increase their chances of qualifying the CSIR NET Life Sciences examination and embark on a rewarding career in the field of Life Sciences.

Disclaimer: It’s important to note that the above syllabus is a general outline, and specific topics and subtopics within each section may vary from year to year. It is recommended to refer to the official CSIR NET Life Sciences syllabus or consult the official website for the most up-to-date and detailed information.

Frequently Asked Questions(FAQ) And Answers On CSIR NET Life Sciences Syllabus:

1: What is the CSIR NET Life Sciences syllabus?

The CSIR NET Life Sciences syllabus encompasses various topics in life sciences, including subjects such as cell biology, molecular biology, genetics, biochemistry, biotechnology, ecology, evolution, and behavior. The syllabus is designed to test the knowledge and understanding of these fundamental concepts and their applications in the field.

2: Is the CSIR NET Life Sciences syllabus the same every year?

The core topics and subjects in the CSIR NET Life Sciences syllabus remain relatively consistent from year to year. However, minor modifications or updates can occur, reflecting advancements in the field of life sciences. It is advisable to refer to the official CSIR website or the latest information bulletin for the most accurate and up-to-date syllabus.

3: How should I prepare for the CSIR NET Life Sciences exam based on the syllabus?

To prepare for the CSIR NET Life Sciences exam, it is important to thoroughly study and understand the topics mentioned in the syllabus. Utilize standard textbooks, review articles, and study materials specifically designed for CSIR NET preparation. Additionally, practicing previous years’ question papers and taking mock tests can help familiarize yourself with the exam pattern and improve time management skills.

4: Are there any online resources or study materials available for the CSIR NET Life Sciences syllabus?

Yes, there are numerous online resources and study materials available for CSIR NET Life Sciences preparation. Websites such as Examrace, BioTecNika, and Easy Biology Class offer study materials, practice questions, and video lectures specifically tailored for the CSIR NET exam. Additionally, online platforms like Coursera and edX provide free or paid courses covering various topics within the life sciences field.

5: Can the CSIR NET Life Sciences syllabus overlap with other competitive exams like GATE or DBT-JRF?

Yes, there can be some overlap in the syllabi of CSIR NET Lifesciences, GATE (Graduate Aptitude Test in Engineering), and DBT-JRF (Department of Biotechnology Junior Research Fellowship) exams, especially in subjects like molecular biology, genetics, biochemistry, and biotechnology. However, the focus and depth of questions may vary. It is important to check the specific syllabi for each exam to understand the similarities and differences.

6: How important is it to cover the entire syllabus for the CSIR NET Life Sciences exam?

It is crucial to cover the entire syllabus as the CSIR NET Lifesciences exam can include questions from any topic mentioned in the syllabus. While some topics may have more weightage, neglecting any area entirely can be risky. It is advisable to allocate time appropriately and ensure a comprehensive understanding of all the subjects.

How Pharmacists and Chemists Can Become The Key Players in Species Conservation

In the wake of the alarming decline of numerous animal and plant species towards endangerment and extinction, a scientist from the University of Michigan stresses the pressing need for chemists and pharmacists to actively engage in species conservation endeavors because pharmacists and chemists can become the key players in species conservation.

DateApril 30, 2024
SourceUniversity of Michigan
SummaryIn light of the imminent threat of losing numerous animal and plant species to endangerment and extinction, a scientist emphasizes the critical role of chemists and pharmacists in conservation initiatives.
Biology News

If you want to know recent biology news like pharmacists and chemists can become the key players in species conservation., then read here: Specific Genomic Changes in the Monkeypox Virus Associated with Their TransmissibilityBetter View of Living Bacteria with New Mid-Infrared NanoscopyWhy Green-to-Red Transformation of Euglena gracilis is in News.

Why Pharmacists and Chemists Can Become The Key Players in Species Conservation:

  • Timothy Cernak, assistant professor of medicinal chemistry at the U-M College of Pharmacy, emphasizes the critical need for medicinal chemistry expertise on the forefront of extinction becausepharmacists and chemists can become the key players in species conservation.
  • He highlights that while animals are facing alarming rates of mortality, there is hope through the application of modern bioscience breakthroughs, originally developed for human disease treatment, in wildlife conservation efforts so pharmacists and chemists can become the key players in species conservation.
  • He emphasized that they are currently experiencing a widespread extinction event, with mass mortality events occurring globally. From lowland gorillas to Argentinian penguins, and the akikiki bird in Hawaii to loggerhead turtles in Florida, the list is extensive, with numerous precious plant species also teetering on the brink.
  • With deadly fungus devastating Panamanian golden frogs, cancerous tumors afflicting loggerhead turtles, and a multitude of pests and diseases plaguing plants like the hemlock tree, conservation medicine faces a plethora of challenges to address.

Watch The Video of An Extinct Animal Here

Causes of Extinction:

According to Cernak, the ongoing mass extinction is propelled by factors such as habitat destruction, climate change, and excessive harvesting. However, one particular underlying issue is wildlife diseases, which presents a promising opportunity for intervention. Medicinal chemistry stands as that intervention and pharmacists and chemists can become the key players in species conservation.

ResearchObservationConclusion
In one of his numerous roles and research endeavors, Cernak receives specimens of deceased and ailing species from various regions worldwide.

Employing methodologies and models akin to those utilized in identifying compounds effective against human diseases, his laboratory at U-M, recently augmented by the addition of a veterinarian, assesses chemical compounds’ efficacy on these samples to identify those that combat disease-causing organisms.

A significant emphasis is placed on addressing fungus, which stands as the primary threat to amphibians, causing widespread mortality


They suggest that while a lasting solution to mass extinction lies in addressing climate change and habitat loss through innovative technologies and policies, there is an immediate need for chemistry to aid endangered species as a short-term measure.
Medicinal chemists invested in extinction prevention are urged to engage in discussions with a range of professionals, including zookeepers, foresters, veterinarians, entomologists, wildlife rehabilitators, and conservationists.

Research

Cernak’s laboratory has pioneered the application of artificial intelligence and other technologies to accelerate the drug discovery process. He emphasized that this advancement not only enhances the potential to assist animals and plants but also expedites the timeframe for providing such aid because pharmacists and chemists can become the key players in species conservation.

FAQ:

1. What is endangerment and extinction?

Endangerment refers to the state of being at risk of extinction, where a species faces significant decline in population and is threatened with disappearing entirely. Extinction occurs when a species no longer exists anywhere in the world.

2. How serious is the issue of endangerment and extinction?

The issue is extremely serious. Scientists warn that we are currently experiencing a mass extinction event, with species disappearing at an alarming rate. This threatens biodiversity, disrupts ecosystems, and can have cascading effects on human well-being.

How Hybrid Brains Help Mice To Smell Like A Rat | Biology News

The researchers began their experiment by identifying mice that lacked a functional olfactory system, rendering them unable to perceive smells. These mice served as the subjects for the experiment, providing a unique opportunity to investigate the restoration of sensory function. This marks the first successful integration of sensory apparatus from one species into another. Thus researchers proves that hybrid brains help mice to smell like a rat.

DateApril 25, 2024
SourceColumbia University Irving Medical Center
SummaryScientists have achieved a breakthrough by restoring the sense of smell in mice that were previously without an olfactory system, using neurons from rats these hybrid brains help mice to smell like a rat
Biology News

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Experiment: Hybrid Brains Help Mice To Smell Like A Rat:

ResearchObservationConclusion

The research team implanted rat stem cells into mouse blastocysts, a developmental stage that occurs shortly after fertilization.

This allowed the rat and mouse cells to coalesce and naturally integrate with each other.
In their initial hybrid experiments, the team investigated the distribution of rat neurons within the mouse brain.

Despite rats typically having larger brains and a slower developmental pace, the rat cells conformed to the developmental cues of the mouse, speeding up their maturation process and forming analogous connections to those observed in native mouse cells.

They observed rat cells spread across nearly the entirety of the mouse brain was quite unexpected

This indicates that there are minimal obstacles to integration, indicating that various types of mouse neurons could potentially be substituted by equivalent rat neurons.

The researchers then assessed whether the rat neurons had been incorporated into a functional neural circuit, specifically within the olfactory system, crucial for mice to locate food and evade predators.


By manipulating the mouse embryo to eliminate or deactivate its own olfactory neurons, the researchers could easily determine if the rat neurons had reinstated the animals’ sense of smell.
Experiment

Result of The Experiments:

They placed a cookie in each mouse cage and observed their ability to locate it using the rat neurons. However, some mice performed better than others in finding the cookie. That is hybrid brains help mice to smell like a rat.

The researchers discovered that mice retaining their own silenced olfactory neurons were less adept at finding hidden cookies compared to those whose olfactory neurons were engineered to vanish during development.

If you want to read more such biology news: Why Fasting is Not Always Good for Your Health, Cell Membrane Damage Promotes Cellular Senescence.

The hybrid brains help mice to smell like a rat. This indicates that adding replacement neurons isn’t a simple plug-and-play process. For functional replacement, it may be necessary to clear out dysfunctional neurons that are inactive, which could be relevant in certain neurodegenerative diseases as well as neurodevelopmental disorders like autism and schizophrenia.

FAQ:

1. What does it mean for a mouse to lack an olfactory system?

Mice without an olfactory system are unable to smell due to a malfunction or absence of the olfactory organs and related neural pathways responsible for detecting odors.

2. What are the future implications of this research?

The findings that hybrid brains help mice to smell like a rat. pave the way for further exploration of brain plasticity and neural engineering. They offer hope for developing innovative treatments for individuals with sensory deficits and neurological disorders, ultimately improving their quality of life.

3. Are there any challenges in the process of restoring the sense of smell in mice?

Yes, one challenge is ensuring the functional integration of the transplanted neurons into the existing neural circuits. Researchers also note that successful integration may require the removal of dysfunctional neurons, indicating that the process is not simply “plug-and-play.”

Scientists Reveal That PI3K Enzyme Has Both Accelerator And Brake Functions

A team of researchers has advanced our understanding of a crucial enzyme governing cell migration. Their recent publication in the journal Nature Communications highlights that the PI3K enzyme has both accelerator and brake functions.

DateApril 24, 2024
SourceTohoku University’s Frontier Research Institute for Interdisciplinary Sciences (FRIS)
SummaryThe enzyme PI3K is crucial for cell migration, a function long recognized by scientists. However, recent findings reveal that a subunit of this enzyme can also act as a brake, halting this process.
Biology News

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About PI3K:

The phosphoinositide 3-kinase (PI3K) is a significant signaling enzyme extensively studied for over three decades due to its pivotal roles in essential cellular functions such as growth, survival, movement, and metabolism. It also plays a crucial role in cell migration and invasion, processes whose dysregulation can lead to various pathologies.

PI3K Enzyme Has Both Accelerator And Brake Functions:

ResearchObservationConclusion
Employing a blend of bioinformatics, molecular modeling, biochemical binding assays, and live-cell imaging, researchers disordered segment within the inter-SH2 domain of p85β directly interacts with the endocytic protein AP2.

This segment of PI3K can trigger a cellular process that draws specific molecules into the cell, independent of the enzyme’s usual lipid-modification function.


Disrupting this binding resulted in the mutated p85β failing to function properly.

Instead of modulating cell movement through its brake mechanism, it accumulated in specific cellular locations.

Instead of modulating cell movement through its brake mechanism, it accumulated in specific cellular locations.

This led to heightened and sustained cell movement, indicating a loss of control over cell migration by the brake mechanism.

Significantly, this single PI3K enzyme has both accelerator and brake functions within its molecular structure.

The endocytic mechanism plays a pivotal role in regulating PI3K’s activity, ensuring that cell movement is appropriately controlled during critical biological processes.
Research

Relation of PI3K Enzyme With Cancer:

  1. Instead, the scientists reveal PI3K enzyme has both accelerator and brake functions but the braking function was identified as unique to the p85β subunit alone.
  2. The p85β subunit of PI3K is associated with cancer-promoting characteristics, gaining a deeper comprehension of PI3K regulation and its isoform specificity could pave the way for innovative therapeutic approaches.
  3. These strategies could selectively target and inhibit the cancer-promoting attributes of PI3K while safeguarding the normal functions of PI3K in healthy cells.

If you want to read more such biology news: Why Fasting is Not Always Good for Your Health, Cell Membrane Damage Promotes Cellular Senescence.

While the PI3K enzyme has both accelerator and brake functions, the ongoing research on PI3K aims to deepen our understanding of PI3K signaling and develop more effective and safer therapies. Combining PI3K inhibitors with other cancer treatments shows promise in overcoming drug resistance and improving patient outcomes.

FAQ:

1. What is the structure of the PI3K enzyme?

PI3K (Phosphoinositide 3-Kinase) is composed of two subunits: a regulatory subunit (p85) and a catalytic subunit (p110). These subunits work together to form a functional enzyme.

2. What does each subunit of PI3K do?

The p85 subunit helps regulate the activity of the enzyme, while the p110 subunit carries out the catalytic function, adding phosphate groups to specific lipids in the cell membrane.

3. Are there different types of PI3K?

Yes, PI3K is classified into several classes and isoforms, each with specific functions and tissue distributions. These variations contribute to the diverse roles of PI3K in different cell types.

Why T. rex Was Not As Smart As Previously Claimed | Biology News

T. rex was not as smart as previously claimed is in top science news because the latest research, released today in The Anatomical Record, conducted by a collaborative team including the University of Bristol, delves into the methodologies for predicting brain size and neuron counts in dinosaur brains. The findings highlight the unreliability of previous assumptions regarding the brain size and neuron count in dinosaurs.

DateApril 29, 2024
SourceUniversity of Bristol
SummaryPrevious research suggests that dinosaurs possessed intelligence comparable to reptiles but fell short of the level seen in monkeys.
Biology News

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About T. rex:

  • Tyrannosaurus rex (T. rex) lived approximately 68 to 66 million years ago, during the Late Cretaceous period, in what is now North America.
  • With its massive size, powerful jaws, and distinctive appearance, it belonged to a group of carnivorous dinosaurs known as theropods, characterized by their bipedal stance, sharp teeth, and predatory behavior.
  • T. rex stood tall, reaching heights of up to 20 feet at the hips and lengths of around 40 feet from head to tail.
  • Estimates suggest that it weighed between 9 and 14 metric tons, making it one of the largest land predators ever known.

Previous Research:

  • A study published last year asserted that dinosaurs such as T. rex possessed an unusually large number of neurons, indicating higher intelligence than previously believed.
  • This research suggested that these elevated neuron counts could offer insights into intelligence, metabolism, and life history, portraying T. rex as exhibiting behaviors reminiscent of primates.
  • Additionally, the study suggested that T. rex might have displayed cognitive abilities such as cultural transmission of knowledge and potential tool use.

Watch The Video of The Dinosaur Here

Research: Why T. rex Was Not As Smart As Previously Claimed:

ResearchObservationConclusion
This study builds upon decades of analysis conducted by paleontologists and biologists who have scrutinized the size and anatomy of dinosaur brains. These researchers have utilized data from mineral infillings within brain cavities, known as endocasts, as well as the shapes of these cavities, to glean insights into dinosaur behavior and lifestyle.The team observed that the estimated brain size, particularly that of the forebrain, had been exaggerated, consequently affecting neuron counts. Furthermore, they demonstrated that using neuron count estimates as a gauge for intelligence is not dependable.The team asserts that to accurately reconstruct the biology of extinct species, and concluded that researchers should consider various lines of evidence, such as skeletal anatomy, bone histology, the behavior of modern relatives, and trace fossils.
Research

The recent scientific research that is T. rex was not as smart as previously claimed has challenged the long-standing perception of Tyrannosaurus rex as a highly intelligent predator. Studies comparing the brain structure of T. rex to other dinosaurs and modern-day birds, its closest living relatives, indicate that its brain-to-body ratio was relatively low.

FAQ on T. rex Was Not As Smart As Previously Claimed

1. How did T. rex become extinct?

The exact reasons for the extinction of T. rex, along with the non-avian dinosaurs, are still debated among scientists. One prevalent theory suggests that a catastrophic event, such as an asteroid impact, led to dramatic environmental changes that ultimately resulted in the extinction of many species, including T. rex.

2. Are there any T. rex fossils on display?

Yes, numerous museums around the world house T. rex fossils and exhibits, allowing visitors to learn about this fascinating dinosaur firsthand. Some notable museums with T. rex displays include the American Museum of Natural History in New York and the Field Museum in Chicago.

Study Reveals An Enhanced Bacterial Defense Against Viral Infection

The study was published on April 16 in Nature Structural & Molecular Biology revealed that one key factor responsible for bacterial defense against viral infection is when phages invade bacteria and exploit their cellular mechanisms for replication. Recent advancements in technology have facilitated the identification of the proteins engaged in these defense mechanisms. However, researchers are still delving further into the functions of these proteins.

DateApril 26, 2024
SourceOhio State University
SummaryScientists document the molecular formation of a prevalent anti-phage system, belonging to the protein family known as Gabija, which is believed to be employed by approximately 8.5% to 18% of bacterial species worldwide.
Biology News

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About Bacteriophages:

Bacteriophages are viruses composed of genetic material, either DNA or RNA, encased in a protein coat. They come in various shapes and sizes, ranging from simple structures to complex arrangements resembling lunar landers. Despite their diversity, all bacteriophages share a common mission: to infect bacterial cells and hijack their cellular machinery to replicate and produce more phages.

Experiment: Bacterial Defense Against Viral Infection

ResearchObservationConclusion
The components of this bacterial defense against viral infection are referred to as Gabija A and Gabija B, abbreviated as GajA and GajB, respectively.

Using cryo-electron microscopy, scientists employed a method to discern the biochemical architectures of GajA and GajB separately, as well as of a supramolecular complex termed GajAB. This complex arises when the two proteins combine, forming a cluster composed of four molecules from each protein.
Scientists observed that while one protein alone dictate the ability to repel a phage, its effectiveness significantly amplifies when it interacts with another protein. This combined complex exhibits remarkable proficiency in cleaving the genome of an invading phage, rendering it incapable of replication.Researchers hypothesize that the formation of the complex between the two proteins is essential for their involvement in phage prevention. However, they also suggest that one protein on its own possesses some anti-phage capabilities.
Experiment

Future Application of The Experiment:

Watch The Application of Bacteria Here

These discoveries of bacterial defense against viral infection contribute to our comprehension of microorganisms’ evolutionary tactics and hold potential for future biomedical applications, according to researchers.

If you want to read more such biology news: Why Fasting is Not Always Good for Your Health, Cell Membrane Damage Promotes Cellular Senescence.

Bacteria employ sophisticated defense mechanisms to combat viral infections. Recent research has unveiled intricate molecular assemblies, such as the Gabija protein complex, which play pivotal roles in bacterial defense against viral infection. Understanding these defense strategies enhances our grasp of microbial biology and may pave the way for innovative biomedical interventions in the future.

FAQ on Bacterial Defense Against Viral Infection:

1. What is the Gabija protein complex?

The Gabija protein complex is a molecular assembly found in bacteria that plays a key role in defending against viruses. It consists of two proteins, Gabija A and Gabija B, which work together to inhibit viral replication.

2. How does the Gabija complex work?

When Gabija A and Gabija B proteins combine, they form a potent defense complex. This complex can disrupt the viral genome, rendering the virus unable to replicate and propagate within the bacterial host.

3. Why is understanding bacterial defense important?

Understanding how bacteria defend against viral infections provides insights into fundamental biological processes. Moreover, this knowledge may inspire the development of novel strategies for combating bacterial infections and advancing biomedical research

Intermittent Fasting Provides Defense Against Liver Inflammation and Liver Cancer

Intermittent fasting provides defense against liver inflammation and liver cancer because an approved drug for these diseases can partially imitate the effects of intermittent fasting. Fatty liver disease frequently progresses to chronic liver inflammation and may result in liver cancer. Recent studies in mice demonstrate that intermittent fasting, following a 5:2 schedule, can effectively arrest this progression. This fasting regimen diminishes the incidence of liver cancer in mice already afflicted with liver inflammation.

DateMay 7, 2024
SourceGerman Cancer Research Center (Deutsches Krebsforschungszentrum, DKFZ)
SummaryResearchers have pinpointed two proteins within liver cells that collaborate to engender the protective benefits of fasting. Furthermore, an approved medication exhibits the capacity to emulate this effect to some extent.
Biology News: Intermittent Fasting Provides Defense Against Liver Inflammation and Liver Cancer

If you want to know recent biology news like Intermittent Fasting Provides Defense Against Liver Inflammation and Liver Cancer, then read here: Why Fasting is Not Always Good for Your Health, Specific Genomic Changes in the Monkeypox Virus Associated with Their TransmissibilityBetter View of Living Bacteria with New Mid-Infrared Nanoscopy.

About The Liver Diseases:

The most prevalent chronic liver condition is non-alcoholic fatty liver disease (NAFLD), which can have severe implications. If left unaddressed, NAFLD can progress to liver inflammation, known as metabolic dysfunction-associated steatohepatitis (MASH), liver cirrhosis, and potentially liver cancer. Fatty liver disease is predominantly perceived as a direct consequence of obesity.

Experiment: Intermittent Fasting Provides Defense Against Liver Inflammation and Liver Cancer

Watch Here: What does the liver do?

ExperimentObservationConclusion


The animals(mice) were provided with a diet high in both sugar and fat, resembling the typical Western diet.


One set of mice had unrestricted access to this diet. As anticipated, these mice experienced weight gain, increased body fat, and developed chronic liver inflammation.


During the exploration of various intermittent fasting regimens, it became evident that several factors such as the frequency and duration of fasting cycles, along with the timing of the fasting phase initiation, influence the safeguarding against liver inflammation.

On the other hand, the mice in the alternate group underwent a dietary regimen where they fasted for two days a week for 5:2, while being allowed to eat huge on the remaining days.

Despite consuming a high-calorie diet, these mice did not exhibit weight gain, displayed fewer indications of liver disease, and demonstrated reduced levels of biomarkers associated with liver damage.From this analysis, two key components driving the protective response to fasting were identified: the transcription factor PPARα and the enzyme PCK1. These molecular entities collaborate to enhance the breakdown of fatty acids and gluconeogenesis while impeding the accumulation of fats
Biology News: Intermittent Fasting Provides Defense Against Liver Inflammation and Liver Cancer

Role of Approved Drug:

The medication pemafibrate emulates the actions of PPARα within the cell. Is it possible for this substance to replicate the protective benefits of fasting as well? To address this inquiry, researchers conducted experiments in mice. Pemafibrate prompted certain beneficial metabolic alterations akin to those observed during 5:2 fasting so the researchers can say that the intermittent fasting provides defense against liver inflammation and liver cancer

Intermittent fasting provides defense against liver inflammation and liver cancer through controlled periods of fasting, the body undergoes metabolic shifts that contribute to the reduction of liver inflammation and the inhibition of cancerous growth.

FAQ on Intermittent Fasting Provides Defense Against Liver Inflammation and Liver Cancer:

1. What are PPARα and PCK1?

PPARα (Peroxisome Proliferator-Activated Receptor alpha) and PCK1 (Phosphoenolpyruvate Carboxykinase 1) are two important proteins found in the liver cells of both humans and animals.

2. What do PPARα and PCK1 do?

PPARα plays a crucial role in regulating the expression of genes involved in fatty acid breakdown and energy metabolism in the liver. It helps in the breakdown of fatty acids and promotes their utilization for energy production.
PCK1, on the other hand, is an enzyme involved in a process called gluconeogenesis, where new glucose is produced from non-carbohydrate sources like fats and proteins. It also plays a role in regulating blood sugar levels.